Table 1 Estimated patterns of SARS-CoV-2 fecal viral shedding in wastewater. Note in this study, SARS-CoV-2 RNA concentration was measured using quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays during August 31, 2020, through April 11, 2023, and reverse transcription digital PCR (RT-dPCR) assays from November 1, 2022, through August 29, 2023. Based on samples tested using both assays, the RT-qPCR and RT-dPCR measures differed by a factor of 16.7. We used this conversion factor to convert measures from the two methods and provide estimates for RT-qPCR and RT-dPCR assays, separately
Model | Wave | Shedding rate (billion copies per day per infectious person, mean and 95% Confidence interval) | Lag (days) | Number of samples | Adjusted R2 |
|---|---|---|---|---|---|
Include all variant waves | 2nd wave (08/31/20-06/26/21) | 1.44 (1.35, 1.53) per qPCR; 24.0 (22.49, 25.51) per dPCRa | 0 | 3 | 0.84 |
Delta wave (06/27/21-12/04/21) | 1.13 (0.86, 1.4) per qPCR; 18.9 (14.45, 23.35) per dPCRa | 0 | 3 | 0.84 | |
Omicron period (12/05/21-08/29/23) | 0.6 (0.59, 0.61) per qPCR; 9.96 (9.76, 10.16) per dPCRb | 0 | 3 | 0.84 | |
Stratified by wave/ period | 2nd wave (08/31/20-06/26/21) | 1.44 (1.37, 1.52) per qPCR; 24.07 (22.85, 25.28) per dPCRa | 0 | 4 | 0.74 |
Delta wave (06/27/21-12/04/21) | 1.09 (0.91, 1.27) per qPCR; 18.14 (15.21, 21.08) per dPCRa | 鈭�5 | 4 | 0.37 | |
Omicron period (12/05/21-08/29/23) | 0.6 (0.59, 0.61) per qPCR; 9.98 (9.76, 10.2) per dPCRb | 0 | 3 | 0.86 | |
Omicron BA.1 (12/05/21-03/05/22) | 0.59 (0.56, 0.61) per qPCR 9.78 (9.32, 10.23) per dPCRa | 0 | 3 | 0.91 | |
After BA.1 (03/06/22-08/29/23) | 0.72 (0.7, 0.75) per qPCR; 12.11 (11.72, 12.5) per dPCRb | 鈭�5 | 4 | 0.78 |